Human Fibronectin

GWB-F286B0

Purified Proteins and Bioactive Peptides

0. 1M Tris-HCl pH 7. 4 containing 0. 15M Sodium chlorideApplications Notes : Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces including plasticware glassware and microcarrier beads. After spin and opening of the vial completely thaw the tube at 37C without agitation flicking or mixing then gently mix the contents with a pipette. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

220kDa reducing conditions

yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Secreted.

The alpha chain is not glycosylated.

About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.

Defects in FGA are a cause of congenital afibrinogenemia [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations.

Defects in FGA are a cause of amyloidois type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1 fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome arterial hypertension hepatosplenomegaly cholestasis petechial skin rash.