Myelin Basic Protein

GWB-92BD80

Purified Neuroscience Reagents

Not applicableApplications Notes : Immunoreactivity is checked using polyclonal and monoclonal antibodies against MBP. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination maybe as components of transcriptional complexes and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers with each of them potentially having a specialized function. Induces T-cell proliferation.

Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.

Several charge isomers of MBP; C1 (the most cationic least modified and most abundant form) C2 C3 C4 C5 C6 C7 C8-A and C8-B (the least cationic form); are produced as a result of optional PTM such as phosphorylation deamidation of glutamine or asparagine arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H) C8-A(L) C8-B(H) and C8-B(L) (H) standing for higher and (L) for lower molecular weight. C3 C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging making the protein more cationic.

Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

Belongs to the myelin basic protein family.