Phospho-MDM2(S395) Antibody

Synthetic Peptide

MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein
p53, diminishing its tumor suppressor function. This protein has E3
ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

Polyclonal

4193

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Dot blot analysis of anti-Phospho-MDM2-pS395 Antibody (Cat.#AP3579a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

1:500

55233

This MDM2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S395 of human MDM2.