Comparison

CD14 Antibody (C-term)

Item no. ABC-AP6294B
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, membrane-bound form, urinary form
Available
Primary Accession
P08571
Antigen Type
Synthetic Peptide
Application
FC, WB, IHC-P
Bio Background
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
Bio References
Donati, M., J. Periodontol. 79 (3), 517-524 (2008)
Yuan, F.F., Immunol. Cell Biol. 86 (3), 268-270 (2008)
Setoguchi, M., Biochim. Biophys. Acta 1008 (2), 213-222 (1989)
Goyert, S.M., Science 239 (4839), 497-500 (1988)
Clonality
Polyclonal
Clone/Animal Names
RB14107
Gene ID
929
Gene Name
CD14
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term) (Cat.#AP6294b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
CD14 Antibody (C-term) (Cat.#AP6294b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term) (Cat.#AP6294b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:10~50
Legend image 4
CD14 Antibody (C-term) (Cat. #AP6294b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Isotype
Rabbit Ig
Calculated MW
40076
Antigen Region
292-322
Antigen Source
HUMAN
Target/Specificity
This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.
NCBI Accession
NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
available

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