Glypican 3 (GPC3) Antibody (N-term)

Synthetic Peptide

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

Polyclonal

2719

Purified Rabbit Polyclonal Antibody (Pab)

Anti-GPC3 Antibody (C35) at 1:2000 dilution + Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 66 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

GPC3 Antibody(C35) (cat# AP6337a) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

1:1000

IHC-P

Rabbit Ig

21-50

This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human Glypican 3 (GPC3).