CASP8 Antibody (C-term)

Synthetic Peptide

CASP8 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases.

Polyclonal

841

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-CASP8 Antibody (C-term) at 1:2000 dilution
Lane 1: BA/F3 whole cell lysate
Lane 2: C2C12 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 55 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

FC

Rabbit Ig

432-461

This CASP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human CASP8.