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SPHK1 Antibody (Center)

SPHK1 Antibody (Center)

Item no.	ABC-AP7237c
Manufacturer	Abcepta
Amount	200 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SPHK1, SPK, SPHK, Sphingosine kinase 1, SK 1, SPK 1
Available
Primary Accession	Q9NYA1
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Bio References	Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). Nava, V.E., et al., FEBS Lett. 473(1):81-84 (2000). Melendez, A.J., et al., Gene 251(1):19-26 (2000). Pitson, S.M., et al., Biochem. J. 350 Pt 2, 429-441 (2000).
Clonality	Polyclonal
Clone/Animal Names	RB03621
Gene ID	8877
Gene Name	SPHK1 (HGNC:11240)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	Anti-SPHK1 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of lysates from 293, HepG2, HUVEC, Raji, rat C6, mouse C2C12 cell line (from left to right), using SPHK1 Antibody (R301)(Cat. #AP7237c). AP7237c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	All lanes : Anti-SPHK1 Antibody (Center) at 1:2000 dilution Lane 1: human liver lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	AP7237c staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 4	IHC-P
Dilution image 4	1:25
Legend image 5	AP7237c staining SPHK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 5	IHC-P
Dilution image 5	1:25
Isotype	Rabbit Ig
Calculated MW	42518
Antigen Region	286-315
Antigen Source	HUMAN
Target/Specificity	This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
NCBI Accession	NP_001136073.1; NP_001136074.1; NP_068807.2; NP_892010.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ul

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Delivery expected until 11/6/2025

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