SPHK1 Antibody (N-term P74)

Synthetic Peptide

Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Polyclonal

8877

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate(from left to right), using SPHK1 Antibody (N-term P74)(Cat. #AP7237d). AP7237d was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

1:1000

IHC-P

Immunohistochemical analysis of paraffin-embedded M. kidney section using SPHK1 Antibody(N-term P74) (Cat#AP7237d). AP7237d was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

1:100

42518

HUMAN

NP_001136073.1; NP_001136074.1; NP_068807.2; NP_892010.2