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PCSK9 Antibody (N-term)

PCSK9 Antibody (N-term)

Item no.	ABC-AP7333a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PCSK9, NARC1, NARC-1, Neural apoptosis-regulated convertase 1, PC9, Proprotein convertase 9, Proprotein convertase subtilisin/kexin type 9, Subtilisin/kexin-like protease PC9, 3421-
Available
Primary Accession	Q8NBP7
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Bio References	Abifadel, M., Rabes, J.P. Hum. Mutat. 30 (7), E682-E691 (2009) McNutt, M.C., Kwon, H.J. J. Biol. Chem. 284 (16), 10561-10570 (2009) Shioji, K., Mannami, T. J. Hum. Genet. 49 (2), 109-114 (2004) Abifadel, M., Varret, M. Nat. Genet. 34 (2), 154-156 (2003)
Clonality	Polyclonal
Clone/Animal Names	RB19130
Gene ID	255738
Gene Name	PCSK9
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term)(Cat. #AP7333a). AP7333a was diluted at 1:2000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of lysate from SW480 cell line, using PCSK9 Antibody (N-term)(Cat. #AP7333a). AP7333a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 2	WB
Dilution image 2	1:500-1:1000
Legend image 3	Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody (N-term)(Cat. #AP7333a). AP7333a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of PCSK9 Antibody (N-term) (Cat. #AP7333a) in Jurkat cell line lysates (35ug/lane). PCSK9 (arrow) was detected using the purified Pab.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Immunohistochemical analysis of paraffin-embedded H. colorectal carcinoma section using PCSK9 Antibody (N-term) (Cat#AP7333a). AP7333a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Type image 5	IHC-P
Dilution image 5	1:25
Legend image 6	PCSK9 Antibody (N-term) (RB19130) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCSK9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 6	IHC-P
Dilution image 6	1:10~50
Legend image 7	Flow cytometric analysis of A431 cells using PCSK9 Antibody (N-term) (green, Cat#AP7333a) compared to an isotype control of rabbit IgG(blue). AP7333a was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Type image 7	FC
Dilution image 7	1:25
Isotype	Rabbit Ig
Calculated MW	74286
Antigen Region	153-183
Antigen Source	HUMAN
Target/Specificity	This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-183 amino acids from the N-terminal region of human PCSK9.
NCBI Accession	NP_777596.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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