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VTN Antibody (N-term)

VTN Antibody (N-term)

Item no.	ABC-AP7462a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	VTN, Vitronectin, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VN
Available
Primary Accession	P04004
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, FC
Bio Background	VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Bio References	Jenne D.E., Stanley K.K.EMBO J. 4:3153-3157(1985) Sigurdardottir O., Wiman B.Biochim. Acta 1208:104-110(1994) Seiffert D., Loskutoff D.J.J. Biol. Chem. 266:2824-2830(1991)
Clonality	Polyclonal
Clone/Animal Names	RB17843
Gene ID	7448
Gene Name	VTN
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-VTN Antibody (N-term) at 1:32000 dilution + human plasma lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:32000
Legend image 2	Anti-VTN Antibody (N-term) at 1:2000 dilution + Caco2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Overlay histogram showing MCF-7 cells stained with AP7462a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP7462a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 4	FC
Dilution image 4	1:25
Isotype	Rabbit IgG
Calculated MW	54306
Antigen Region	65-93
Antigen Source	HUMAN
Target/Specificity	This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-93 amino acids from the N-terminal region of human VTN.
NCBI Accession	NP_000629.3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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