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ACAT1 Antibody (C-term)

ACAT1 Antibody (C-term)

Item no.	ABC-AP7560b
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ACAT1, mitochondrial, MAT, ACAT, T2, Acetyl-CoA acetyltransferase, Acetoacetyl-CoA thiolase
Available
Primary Accession	P24752
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Bio References	Locke, J.A., Prostate 68 (1), 20-33 (2008) Guo, Z.Y., Biochemistry 46 (35), 10063-10071 (2007) Haapalainen, A.M., Biochemistry 46 (14), 4305-4321 (2007)
Clonality	Polyclonal
Clone/Animal Names	RB14644
Gene ID	38
Gene Name	ACAT1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of anti-ACAT1 Pab (Cat.#AP7560b) in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) (Cat.#AP7560b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Other Accession	Q8HXY6
Predicted	Pr
Isotype	Rabbit Ig
Calculated MW	45200
Antigen Region	296-329
Antigen Source	HUMAN
Target/Specificity	This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
NCBI Accession	NP_000010.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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