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FGFR4 Antibody (N-term)

FGFR4 Antibody (N-term)

Item no.	ABC-AP7639a
Manufacturer	Abcepta
Amount	200 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	FGFR4, JTK2, TKF, Fibroblast growth factor receptor 4, CD334, FGFR-4
Available
Primary Accession	P22455
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.
Bio References	References for protein: 1.Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). 2.Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). 3.Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). 4.Becker, N., et al., Cancer Epidemiol. Biomarkers Prev. 12(6):582-583 (2003). 5.Takenaka, H., et al., Arch. Dermatol. Res. 294(7):331-338 (2002). References for HeLa cell line: 1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828]. 2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199]. 3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234]. 4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].
Clonality	Polyclonal
Clone/Animal Names	RB38171
Gene ID	2264
Gene Name	FGFR4
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Anti-FGFR4 Antibody (N-term) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-FGFR4 Antibody (N-term) at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Anti-FGFR4 Antibody (E39) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 4	IHC-P
Dilution image 4	1:50~100
Legend image 5	Flow cytometric analysis of WiDr cells using FGFR4 Antibody (N-term) (bottom histogram) compared to a negative control(top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Isotype	Rabbit Ig
Calculated MW	87954
Antigen Region	24-55
Antigen Source	HUMAN
Target/Specificity	This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4.
NCBI Accession	NP_002002.3; NP_075252.2; NP_998812.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 200 ul

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Delivery expected until 11/6/2025

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