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SMAD4 Antibody (T277)

SMAD4 Antibody (T277)

Item no.	ABC-AP7753a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IF
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SMAD4, Smad4, DPC4, MADH4, MAD homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, SMAD 4, hSMAD4
Available
Primary Accession	Q13485
Antigen Type	Synthetic Peptide
Application	IF, WB
Bio Background	SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.
Bio References	Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004). Horvath, L.G., et al., Prostate 59(3):234-242 (2004). Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004). Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004). Maru, D., et al., Oncogene 23(3):859-864 (2004).
Clonality	Polyclonal
Clone/Animal Names	RB14714
Gene ID	4089
Gene Name	SMAD4
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Confocal immunofluorescent analysis of SMAD4 Antibody (T277)(Cat#AP7753a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-SMAD4 Antibody (T277) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Anti-SMAD4 Antibody (T277) at 1:2000 dilution + Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	Western blot analysis of SMAD4 Antibody (T277)(arrow) using rabbit polyclonal SMAD4 Antibody (T277) (Cat.#AP7753a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2) (Origene Technologies).
Type image 4	WB
Dilution image 4	1:1000
Other Accession	O70437; P97471
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	60439
Antigen Region	255-284
Antigen Source	HUMAN
Target/Specificity	This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.
NCBI Accession	NP_005350.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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