« Back
Home /
MAP2K2 Antibody (S222)

MAP2K2 Antibody (S222)

Item no.	ABC-AP7961e
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MAP2K2, MEK2, Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MKK2, PRKMK2, MAP kinase kinase 2, MAPKK 2, MEK 2
Available
Primary Accession	P36507
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Bio References	Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003). Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003). Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003). Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). Liu, X., et al., J. Biol. Chem. 277(42):39312-39319 (2002).
Clonality	Polyclonal
Clone/Animal Names	RB12322
Gene ID	5605
Gene Name	MAP2K2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of MAP2K2-S222 (Cat. #AP7961e) in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222 (Cat. #AP7961e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) .
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	All lanes : Anti-MAP2K2 Antibody (S222) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) (Cat.#AP7961e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Other Accession	P36506; Q63932; Q90891; Q05116; Q01986; P29678; P31938; Q02750; Q63980; Q24324
Predicted	D, Ha, M, Rb, Rat, X, C
Isotype	Rabbit Ig
Calculated MW	44424
Antigen Region	200-229
Antigen Source	HUMAN
Target/Specificity	This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2.
NCBI Accession	NP_109587.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

ABC-AP7961e-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 400 ul

available

Delivery expected until 11/6/2025

Express Delivery: Save 13 days

Compare

Add to wishlist

Get an offer