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HK2 (Hexokinase II) Antibody (Center)

HK2 (Hexokinase II) Antibody (Center)

Item no.	ABC-AP8140f
Manufacturer	Abcepta
Amount	400 ul
Category	Metabolism Glucose Homeostasis Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Citations	Lehto, M., et al., Diabetologia 38(12):1466-1474 (1995). Vidal-Puig, A., et al., Diabetes 44(3):340-346 (1995). Laakso, M., et al., Diabetes 44(3):330-334 (1995). Echwald, S.M., et al., Diabetes 44(3):347-353 (1995). Shinohara, Y., et al., Cancer Lett. 82(1):27-32 (1994).
NCBI	NP_000180.2
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Hexokinase-2,Hexokinase type II,HK II,Muscle form hexokinase,HK2
Similar products	HK2, Hexokinase-2, Hexokinase type II, Muscle form hexokinase, HK II
Available
Manufacturer - Category	Crown Antibodies; Primary Antibodies; Cancer; Cardiovascular; Metabolism; Signal Transduction; Mitochondrion; Mitochondrion outer membrane
Manufacturer - Targets	This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-483 amino acids from the Central region of human HK2 (Hexokinase II).
Bio Background	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
Clonality	Polyclonal
Polyclonal Antibody Additonal Information	RB5333
Gene Name	HK2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Calculated Molecular Weight	102380
Antigen Source	HUMAN
Antigen Region	453-483
Formulation	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Dilution	WB: 1:1000 IHC-P: 1:100 IHC-P: 1:100
Gene Summary	Overlay histogram showing Hela cells stained with AP17735c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP17735c, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antigen Type	Synthetic Peptide

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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