MyD88 Antibody (Center)

Synthetic Peptide

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. It acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response and increases IL-8 transcription. It may be involved in myeloid differentiation.

Polyclonal

4615

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-MyD88 Antibody (Center) at 1:2000 dilution + HT-29 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 33 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P

Overlay histogram showing K562 cells stained with AP8521C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8521C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

0.059027777777778

Pr

33233

HUMAN

NP_001166037.1; NP_001166039.1; NP_001166040.1; NP_002459.2