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HLA-B Antibody (N-term)

HLA-B Antibody (N-term)

Item no.	ABC-AP8616a
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HLA-B, HLA class I histocompatibility antigen, HLAB, B-27 alpha chain, MHC class I antigen B*27
Available
Primary Accession	P03989
Antigen Type	Synthetic Peptide
Application	IHC-P-Leica, FC, WB
Bio Background	HLA-B is involved in the presentation of foreign antigens to the immune system.
Bio References	Higgins, C.M., et.al., Ann. Rheum. Dis. 51 (7), 855-862 (1992) Guild, B.C.et.al., J. Biol. Chem. 259 (14), 9235-9240 (1984) Orr, H.T., et.alt., Biochemistry 18 (25), 5711-5720 (1979)
Clonality	Polyclonal
Clone/Animal Names	RB20998
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-HLA-B Antibody (N-term) at 1:1000-2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Ramos whole cell lysate Lane 5: A375 whole cell lysate Lane 6: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000-2000
Legend image 2	Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP8616a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Type image 2	IHC-P-Leica
Dilution image 2	1:500
Legend image 3	Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green, Cat#AP8616a) compared to an isotype control of rabbit IgG (blue). AP8616a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Type image 3	FC
Dilution image 3	1:25
Other Accession	P30490; P30488; P30487; P30486; P30485; P30483; P30481; P30479; Q04826; P18463; P30464; P30461
Isotype	Rabbit IgG
Antigen Region	62-90
Antigen Source	HUMAN
Target/Specificity	This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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