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HP Antibody (Center)

HP Antibody (Center)

Item no.	ABC-AP8929c
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HP, Haptoglobin, Zonulin, Haptoglobin beta chain, Haptoglobin alpha chain
Available
Primary Accession	P00738
Antigen Type	Synthetic Peptide
Application	FC, WB, IHC-P
Bio Background	HP is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.
Bio References	Ryndel, M., et.al., Clin. Chim. Acta 411 (7-8), 500-504 (2010) Igl, W., PLoS Genet. 6 (1), E1000798 (2010)
Clonality	Polyclonal
Clone/Animal Names	RB21463
Gene ID	3240
Gene Name	HP
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of HP (arrow) using rabbit polyclonal HP Antibody (Center) (Cat. #AP8929c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HP gene (Lane 2) .
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Anti-HP Antibody (Center) at 1:8000 dilution + Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45, 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:8000
Legend image 3	AP8929c staining HP in human epatocarcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:25
Legend image 4	HP Antibody (Center) (Cat. #AP8929c) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	45205
Antigen Region	295-322
Antigen Source	HUMAN
Target/Specificity	This HP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-322 amino acids from the Central region of human HP.
NCBI Accession	NP_001119574.1; NP_005134.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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