EIF4A2 Antibody (C-term)

Synthetic Peptide

Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins. Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2. The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive amino acid similarity and contain the asp-glu-ala-asp (DEAD) sequence. DEAD-box genes have been identified in species ranging from E-coli to humans. Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).

Polyclonal

1974

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of EIF4A2 Antibody (C-term) (Cat. #AP9190b) in K562 cell line lysates (35ug/lane). EIF4A2 (arrow) was detected using the purified Pab.

1:1000

WB

Anti-EIF4A2 Antibody (C-term) at 1:4000 dilution + MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 46 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:4000

IHC-P

EIF4A2 Antibody (C-term) (Cat. #AP9190b) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

B, Pr, M, Rb, C, Rat

46402

HUMAN

NP_001958.2