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Question 1: Can I change the dilution volume of the experimental lysate being assayed?
Answer 1: Yes, different volumes of lysate can be used to quantify protein levels. The equation to determine protein concentration in µ, g/ml is: OD reading x dilution factor x 100 µ, g/ml/cm if a small volume (1.0 ml) cuvette is used. If a 96 well plate is used, the same equation is used with this single modification: 125 µ, g/ml/cm is the value used rather than 100 µ, g/ml/cm.
Question 2: Can this assay be performed using 96 well plates?
Answer 2: Yes, the protein assay can be performed in either small volume cuvettes (1.0 ml) or a 96 well plate.If a 96 well plate is used, the equation to determine protein concentration in µ, g/ml is: OD reading x dilution factor x 125 µ, g/ml/cm.
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
ADV02-B.pdf
ADV02-B-datasheet.pdf
ADV02-B-safety-datasheet.pdf
Delivery expected until 8/7/2025
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