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Question 1: What is the isotype composition of the skeletal muscle actin?
Answer 1: Skeletal muscle actin (CAt. #AKF99) is composed of 99.5% alpha-actin skeletal muscle isoform (Swiss Protein Databank Acc. # P68135.1) .
Question 2: Can the pre-formed actin filaments from rabbit skeletal muscle be used with cardiac myosin from bovine to study myosin&rsquo, s ATPase activity?
Answer 2: Yes, the pre-formed actin filaments prepared from rabbit skeletal muscle (CAt. #AKF99) is recommended to activate the ATPase activity of myosin prepared from bovine cardiac muscle ((Cat. #MY03). The activation of cardiac muscle myosin is much less than the activation observed with rabbit skeletal myosin (Cat. #MY02), i.e. MY03 atpase is activated 3 to 4 fold over it&rsquo, s endogenous, whereas MY02 is activated 50 to 100 fold.
Question 3: Is this product suitable for high resolution microscopic imaging of actin filaments?
Answer 3: No, the pre-formed actin filaments are ideal for activating myosin&rsquo, s ATPase activity, and spin-down binding assays but we do not recommend AKF99 for high resolution microscopic analysis. The reason is that AKF99 preparation involves cycles of lyophilization and freeze/thaw which create denatured zones in the protein. The ability to activate myosin&rsquo, s ATPase activity or bind F-actin accessory proteins is not affected. For high resolution microscopy, we recommend using 99% pure actin monomers (Cat. #AKL99) to polymerize fresh filaments and use them for such analyses.
Question 4: Upon resuspension, what are the buffer components?
Answer 4: The protein will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5 mM MgCl2 and 5% (w/v) sucrose.
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
AKF99-B.pdf
AKF99-B-datasheet.pdf
AKF99-B-safety-datasheet.pdf
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