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Question 1: Which kinesin motor proteins can be used in the Kinesin ELIPA Biochem Kit (Cat. #BK060)?
Answer 1: The Kinesin ELIPA Biochem Kit (Cat. #BK060) can be used with any of the other kinesin motor proteins sold by Cytoskeleton, Inc. Please see the protein&rsquo, s datasheet for representative data detailing each motor protein&rsquo, s ATPase activity using either this kit (Cat. #BK053) or our Kinesin ELIPA Biochem Kit (Cat. #BK060).
Question 2: Are there specific requirements of the spectrophotometer that I will use to measure the inorganic phosphate production with the Kinesin ELIPA Biochem Kit (Cat. #BK060)?
Answer 2: The assay is based upon an absorption shift from 340 nm to 360 nm. It is therefore very important to use a spectrophotometer that has a narrow bandwidth in order that the wavelength for reading the assay does not encroach upon the 340 nm range. It is recommended that a monochromatic spectrophotometer such as a SpectroMax 350 (Molecular Devices) be used when possible as the bandwidth in these machines is very narrow (2-5 nm). If a filter based system is being used then it is important to make sure that the filter bandwidth is 10 nm or less, if not the result will be a) significant background noise and b) greatly reduced sensitivity of the assay. The spectrophotometer should be at room temperature and set on kinetic mode and it is recommended to take a reading once every 30 seconds. There is no need to elect a blank well as the reaction minus motor will serve as a background control. Do not preread the microtiter plate. Start the kinetic readings at time zero.
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
BK060.pdf
BK060-datasheet.pdf
BK060-safety-datasheet.pdf
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