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Question 1: How does general tubulin buffer differ from G-PEM buffer?
Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization. General tubulin buffer ((Cat. #BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0. Both buffers should be supplemented with 1 mM GTP (hence, the &ldquo, G&rdquo, in G-PEM buffer).
Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin?
Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization, however, glycerol is not necessary for the maintenance of biologically active tubulin.
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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