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Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Ultra-Low Endotoxin

Anti-IFN Beta (HDB-4A7) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1110UL-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Western Blot Blocking
Type	Antibody
Applications	WB, Blocking, FA
Clone	HDB-4A7
Specific against	Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG2a
Purity	>98% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	IFN-b, IFN-beta, Type-1 interferon, Interferon beta
Shipping Condition	Cool pack
Available
Specificity	Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant
Manufacturer - Applications	Western Blot, Blocking, Functional Assays
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Mouse IgG2a
Manufacturer - Targets	IFN beta
Shipping Temperature	Blue ice
Storage Conditions	anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Application Notes	Functional Assay: In Vitro Activity of HDB-4A7 - inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). - blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. - neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV In Vivo Activity of HDB-4A7 - circulates with a half-life of ~ 14 days. - blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice. Each investigator should determine their own optimal working dilution for specific applications.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1, 4, 5 or 13); HDb-4A7 binds both recombinant and natural forms
Purification method	This monoclonal antibody was purified using Protein A
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Plasmid DNA encoding murine IFNb
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)
Related Antibodies	Anti-IFN beta In Vivo Antibody - Low Endotoxin [HDB-4A7] (ICH1110)
Isotype Control	Mouse IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [C1.18.4] (ICH2245UL)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 mg

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Delivery expected until 9/18/2025

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