Comparison

Anti-Mouse CD44 (IM7) In Vivo Antibody - Low Endotoxin European Partner

Item no. ICH1115-100MG
Manufacturer IchorBio
Amount 100 mg
Quantity options 100 mg 1 mg 25 mg 50 mg 5 mg
Category
Type Antibody
Applications WB, FC, IP, IHC, ELISA, FA
Clone IM7
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2b
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I
Shipping Condition Cool pack
Available
Specificity Anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186
Manufacturer - Applications
Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen), ELISA, Functional Assays
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2b
Manufacturer - Targets
CD44
Shipping Temperature
Blue ice
Storage Conditions
anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reacts with: Ms+, Hu+, Bov+, Mky+, Bab+, Dog+, Cat, Porcine
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Clone IM7 antibody has been shown to neutralize CD44 in vivo
Concentration
0.5 mg/ml
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Related Antibodies
Isotype Control
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 mg
Available: In stock
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