Comparison

NGS Videos

Erfahren Sie mehr über NGS

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    1) Next Generation Sequencing (NGS) - An Introduction

    Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of DNA molecules simultaneously. This powerful tool is revolutionizing fields such as personalized medicine, genetic diseases, and clinical diagnostics by offering a high throughput option with the capability to sequence multiple individuals at the same time. Learn more about

    • Sanger Sequencing
    • NGS overview
    • Pyrosequencing
    • Sequencing by synthesis and by ligation
    • Comparisons across NGS platforms

    Fitting Products:

    ABM-G947S
    DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
    ABM-IW20150/a>
    Whole Genome Sequencing (5Gb, 2x75bp, PE)
    ABM-IE30501
    Exome Sequencing (Human, 45Mb Exome, 40X Coverage at Raw data level, 30X Average On-target Coverage)
    ABM-IA20001
    Custom 16S Amplicon Primers
    ABM-IW10001
    Full-Length Plasmid Sequencing (100Mb, 2x75bp PE)
    ABM-IW20150/a>
    RecA Protein, E. coli
    ABM-G950
    DNA Purification SPRI Magnetic Beads
    ABM-E016
    Exonuclease III, E. coli
    ABM-E007
    E. coli DNA Ligase
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    2) Next Generation Sequencing (NGS) - Sample Preparation

    Major concepts in NGS (Next generation sequencing) are discussed, such as the science behind it and how to work with sequencing data. In this video, the steps to prepare samples for NGS in the specifics of each sequencing application are outlined. In this installment, the steps of library preparation for Whole Genome Sequencing, Exome Sequencing, RNA Sequencing and Methyl Sequencing on the Illumina platform are shown in detail.

    • Whole genome Sequencing
    • Exome sequencing
    • RNA sequencing
    • Methylation sequencing

    Fitting products:

    ABM-G947S
    DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
    ABM-IW20100/a>
    Whole Genome Sequencing (1Gb, 2x75bp, PE)
    ABM-IE30501
    Exome Sequencing (Human, 45Mb Exome, 40X Coverage at Raw data level, 30X Average On-target Coverage)
    ABM-IR13008
    Total RNA Sequencing (8 million reads, 1x75bp SE)
    ABM-IW19001
    DNA Isolation for NGS
    ABM-IS10001/a>
    Data Storage (per additional 3 months)
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    3) Next Generation Sequencing (NGS) - Coverage & Sample Quality Control

    Next Generation Sequencing (NGS) is a useful tool in deterring the DNA sequences, information which is valuable in further understanding biological processes. Compared to other tools, NGS is flexible and it can be used in different applications. However, there are parameters which have to be considered when running a NGS experiment. Here, the quantitative and qualitative measures needed to ensure a good sample for sequencing are outlined. In this installment the importance of having sufficient coverage and the process of quality control are described. Learn more about:

    • Coverage
    • Sample quality control
    • Quantitative validation
    • Qualitative validation

    Fitting products:

    ABM-G947S
    DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
    ABM-IW19001/a>
    DNA Isolation for NGS
    ABM-IH10200
    HiSeq 4000 High Output (1 Lane, 2x150nt, 400M Reads) Lane Rental
    ABM-IR18001
    Sample and Library QC
    ABM-MasterMix-P
    TaqProbe 2X qPCR MasterMix-ROX
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    4) Next Generation Sequencing (NGS) - Data Analysis

    Next Generation Sequencing (NGS) is a useful tool in deterring the DNA sequences, information which is valuable in further understanding biological processes. In this installment, some of the common formats of NGS raw data and software that can be used for downstream analysis are described. Learn more about advantages of different software for your specific purpose and get a short introduction in bioinformatic data analysis programs. Step by step you are introduced how to deal with valuable NGS data. Get to know more about:

    • Raw data output
    • Sequence Alignment
    • Variant Calling
    • Additional Software & Tools

    Fitting products:

    ABM-IE30503
    Exome Sequencing (Human, 45Mb Exome, 100X Coverage at Raw data level, 75X Average On-target Coverage)
    ABM-IB00015/a>
    Exome-Seq Analysis for 40X Raw data level, 30X Average On-target Coverage
    ABM-IW19001
    DNA Isolation for NGS
    ABM-IS10001
    Data Storage (per additional 3 months)
    ABM-IE30503
    Exome Sequencing (Human, 45Mb Exome, 100X Coverage at Raw data level, 75X Average On-target Coverage)
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    5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing

    Whole Genome Sequencing is a powerful technology that is rapidly gaining popularity as it becomes more and more affordable. With whole genome sequencing, you can assemble genomes de novo, compare the genome of your organism to a reference genome, explore the molecular evolution of a species or population, accurately track pathogen outbreaks, and more. This video illustrates some real examples of whole genome sequencing applications. Learn more about

    • WGS (Whole Genome Sequencing) overview
    • De novo Genome Assembly
    • Pathogen Outbreaks
    • Molecular Evolution

    Fitting products:

    ABM-G934
    Plant Ex-Amp Extraction Buffer Set
    ABM-G947S/a>
    DNA Library Prep Kit for Illumina Sequencing (Purification beads included)
    ABM-IR18001
    DSample and Library QC
    ABM-IW20100
    Whole Genome Sequencing (1Gb, 2x75bp, PE)
    ABM-IB00013
    de novo Assembly (<10Mb Genome, Minimum 100X coverage needed)
    ABM-IB00001
    Reference Genome Import (<10Mb genome)
    ABM-IB00011
    Standard WGS Analysis