Mitomycin C, 5 MG

X1004059-0

Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus . It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems. This product is analkylating agentthat specifically targets the guanine nucleoside sequence 5′ -CpG-3′ . It inhibits DNA synthesis by covalently reacting with DNA, forming crosslinks between complementary strands of DNA. This interaction prevents separation of complementary DNA strands, inhibiting DNA replication. Mitomycin C has strongantitumor activity , especially against Ehrlich ascites tumor cells, and strong bactericidal action against gram-positive and gram-negative bacteria. Mitomycin c role in pluripotent stem cells research expands into the CRISPR/Cas9 revolution to permit efficient gene correction 1 . http://stemcellstm.alphamedpress.org/content/5/1/8.full.pdf+html Pluripotent Stem Cells Generated From b-Thalassemia Fibroblasts Allow Efficient Gene Correction With CRISPR/Cas9.

Soluble in water (100 mg/ml at 25 °C), ethanol (50 mM), methanol (5 mg/ml), DMSO (100 mM), and DMF

Blue-Violet Crystals