CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit

- Assay Buffer
- Chromogen
-Substrate

- 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
- The sample preparation should be carried out on ice.
- If the experiment is not carried out immediately, the samples can be kept at -80C for 1 month.
- In the detection of GST activity in cells, the cell number must be between 3-5 x 106, and the extraction of GST in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
- Pre-test is needed before experiment, two or three samples with large difference should be diluted into different concentrations to test.
- If the results collected within 5 minutes are not linear, or the sample absorbance value is greater than 1, it is suggested that the sample should be diluted with distilled water and the result should be multiplied by the dilution factor.
- The reaction temperature has influence on the result. Keep the temperature at 25CC (for general species) or 37CC (for mammals).

Gel pack with blue ice.

Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The enzymes protect cells against toxicants by conjugating them to glutathione, thereby neutralizing their electrophilic sites, and rendering the products more water-soluble. The glutathione conjugatesare metabolized further to mercapturic acid and then excreted. GST has the activity of GSH-Px, also called non-Se GSH-Px, which has the function of repairing oxidative damage of macromolecule such as DNA, protein and so on. GST catalyzed reaction decreased GSH content, but did not increase GSSG content. Based on their sequence homology, substrate specificity and immunological cross-reactivity, GSTs have been grouped into species-independent classes of isozymes. These classes are comprised of both cytosolic and microsomal enzymes.

Abbkine CheKineTM Glutathione S-Transferase (GST) Colorimetric Assay Kit is designed for detecting GST activity in the sample.