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CheKineTM Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit

CheKineTM Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit

Item no.	ABK-KTB1640-96T
Manufacturer	Abbkine Scientific
Amount	96T
Category	Cell Biology Cell Metabolism Other Assays & Kits Other Kits
Type	Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Applications Notes	GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.
Kit Components	- Assay Buffer - Substrate - GR - H2O2
Features And Benefits	- Determination of GSH-Px activity in serum, plasma, tissue/cell lysates and other biological fluids.
Usage Notes	- 96 Well Clear Flat Bottom UV-Transparent Microplate is needed. - The whole processes need to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution. - Prepare mixed reagents for use, and place on ice. - The measuring process should be operated quickly and only 1 ca. 2 blank well should be needed. - The reaction temperature has influence on the result. Keep the temperature at 25CC (for general species) or 37CC (for mammals).
Storage Instructions	Storage at -20CC and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle. GSH-Px not only specifically catalyzes the reaction between reduced glutathione and ROS to form oxidized glutathione GSSG, thereby protecting the biofilm from ROS damage and maintaining the normal function of cells; it also protects the liver and improves the body immunity, antagonizing the harmful effects of harmful metal ions on the body and increasing the body's ability to resist radiation.
Tag	GSH-Px
Description	Abbkine CheKineTM Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit is designed for detecting GSH-Px activity in the sample.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

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Delivery expected until 9/18/2025

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