Comparison

CheKineTM Thioredoxin Reductase (TrxR) Colorimetric Assay Kit

Item no. ABK-KTB1650-96T
Manufacturer Abbkine Scientific
Amount 96T
Category
Type Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Applications Notes
TrxR can catalyzes the reduction of DTNB by NADPH to generate TNB and NADP+. TNB has a characteristic absorption peak at 412 nm. TrxR activity can be calculated by measuring the increase rate of TNB at 412 nm.
Kit Components
- Assay Buffer
- Chromogen
- TrxR Cofactor
Features And Benefits
- Determination of TrxR activity in serum, plasma, tissue/cell lysates and other biological fluids.
Usage Notes
- The whole process needs to be carried out on ice, and the enzyme activity should be determined on the same day, to avoid repeated freeze-thaw of the homogenate solution.
- In the detection of TrxR activity in cells, the cell number must be between 3-5 x 106, and the extraction of TrxR in cells can be followed by Assay Buffer grinding or ultrasonic treatment, cells cannot be treated by lysate.
Storage Instructions
Storage at -20CC and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TrxR is a NADPH-dependent dimer selenase that includes the FAD domain. It belongs to the pyridine nucleotide-disulfide redoxase family and forms a thioredoxin system with thioredoxin and NADPH. TrxR has similar activity to GR and catalyzes the reduction of GSSG to GSH, which is one of the key enzymes in the glutathione redox cycle.
Tag
TrxR
Description
Abbkine CheKineTM Thioredoxin Reductase (TrxR) Colorimetric Assay Kit is designed for detecting TrxR activity in the sample.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock
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