I kappa B Kinase Detection Set

These polyclonal antibodies can be used for detection of IKKα , IKKβ , IKKγ and IKKε by immunoblot at 0.5 - 2 μg/mL, and by immuncytochemistry (IKKα , IKKβ and IKKγ ) or immunohistochemistry (IKKε ) at 1 - 10 μg/mL, and Immunoflourescence.

Nuclear factor kappa B (NF-κ B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα , and bacteria product LPS. NF-κ B is associated with Iκ B proteins in the cell cytoplasm, which inhibit NF-κ B activity. Iκ B kinase (IKK), which phosphorylates Iκ B and mediates Iκ B degradation and NF-κ B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ ). IKKα and IKKβ interact with each other and both are essential for the NF-κ B activation. IKKα specifically phosphorylates Iκ B-α , while IKKβ phosphorylates both Iκ B-α and Iκ B-β . Another molecule in the IKK complex termed IKKγ (also known as NEMO) interacts with IKKβ and is required for the activation of IKK complex and NF-κ B by LPS, PMA, TNF, and IL-1 stimulation. IKKγ was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κ B activation. IKKε is required for the activation of NF-κ B by PMA and T cell receptors but not by TNFα and IL-1. IKKε message is expressed in a variety of tissues and is inducible by TNFα , IL-1, and LPS.

For images please see PDF data sheet

Antibodies are supplied as affinity chromatography purified IgG.

PBS containing 0.02% sodium azide.

Polyclonal

Optimal dilutions for each application to be determined by the researcher.