Power Eva QPCR SuperMix BioGenomics™ Kit

Kits and Assays / Kits-Cloning, BioGenomics™

4°C/-20°C

Components in this kit are prepared with pure chemicals according to our proprietary technology. Power Eva qPCR SuperMix is a 2x concentration of premix reagent including Hot-start DNA polymerase and second generation Eva Green Dye that is comparable to SYBR Green for its fluorescent property. The included real time buffer has been specially formulated for real-time PCR with high specificity and reproducibility.

Real–time or quantitative PCR (QPCR) allows quantification of DNA, cDNA, or RNA templates. QPCR is based on the detection of a fluorescent reporter molecule that increases as PCR products accumulate with each cycle of amplification. In United States Biological’s Power Eva QPCR SuperMix, a superior green fluorescence DNA-binding dye is used for real-time detection and quantitation of DNA. The Power Eva QPCR SuperMix is a ready-to-use, 2x-concentrated master mix that contains all the reagents (except primers and templates) needed for running quantitative, real-time DNA detection assays, in the double-stranded DNA dye detection format. The passive reference dye ROX is included in a separate tube to make the Power Eva Supermix adaptable for many real-time QPCR platforms. A pair of human beta-actin primers is also included in the kit as a control.

Kit Components:
Power Eva QPCR SuperMix
ROX reference Dye

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.