1-mL Im7 4B Gravity Flow Column, SUMO Protease

The 1-mL Im7 4B Resin Gravity Flow Column Starter Kit provides all essential buffers and reagents necessary for the purification of CL7 tagged proteins. Please select a plasmid and an elution protease. The Im7 1-mL Gravity Flow Column Starter Kit consists of: Equilibration, Lysis, Elution Buffer (100 mL) - 0.5 M NaCl, 20 mM Tris-HCl pH 8.0, 5% Glycerol; Wash Buffer A1 (High Salt) (100 mL) - 2 M NaCl, 20 mM Tris-HCl pH 8.0, 5% Glycerol; Wash Buffer A2 (No Salt) (100 mL) - 0 M NaCl, 20 mM Tris-HCl pH 8.0, 5% Glycerol; Gentle Elution Buffer (100 mL) - 3.6 M MgCl2, 20 mM MES, pH 6.6; 1-mL Im7 6B Resin Gravity Flow Column (1 mL); Elution Protease (1 mg); Plasmid (100 ng; 30 ng/µL); See plasmid guide at TriAltusbioscience.com to select your custom plasmid.