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NIH/3T3 Whole Cell Lysate

NIH/3T3 Whole Cell Lysate

Item no.	LEIN-A195-0.1mg
Manufacturer	Leinco Technologies
Amount	0.1 mg
Category	Cell Lines & Cell Culture Cell Lysates
Type	LYSATE
Specific against	other
Host	Mouse
ECLASS 10.1	32190102
ECLASS 11.0	32190102
UNSPSC	41105330
Available
Manufacturer - Applications	This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Storage Conditions	This whole cell lysate is stable for three months at -20°C
Expiration Date	One year from date of receipt
Concentration	2.0 mg/ml
Formulation	The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Product Preparation	3T3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Cell Line	NIH/3T3 (Embryo)
Tissue Type	Mouse Embryo
Growth Media	DMEM & 10% FBS (Fetal Bovine Serum)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 8/21/2025

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