Comparison

MDA-MB-361 Whole Cell Lysate - 0.1 mg

Item no. LEIN-M1006-0.1mg
Manufacturer Leinco Technologies
Amount 0.1 mg
Category
Type LYSATE
Specific against other
Host Human
ECLASS 10.1 32190102
ECLASS 11.0 32190102
UNSPSC 41105330
Shipping Condition Cool pack
Available
Manufacturer - Applications
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Shipping Temperature
Next Day 2 - 8°C
Storage Conditions
This whole cell lysate is stable for three months at -20°C
Expiration Date
One year from date of receipt
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Product Preparation
MDA-MB-361 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Cell Line
MDA-MB-361 (Human Breast Adenocarcinoma)
Growth Media
DMEM/Hams F12 & 10% NCS (Newborn Calf Serum)
Keywords
Cell Line Lysate, MDA-MB-361 Whole Cell Lysate, MDA-MB-361 Whole Cell, MDA-MB-361

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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