Mouse GM-CSF ELISA Kit

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a cytokine belonging to the GM-CSF family. GM-CSF is secreted by a variety of cell types including macrophages, T cells, mast cells, endothelial cells and fibroblasts. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Mouse GM-CSF is synthesized as a 141 amino acid (aa) residue precursor protein containg a 17 aa hydrophobic signal peptide and the 124 aa residue mature protein. It has two potential N-linked glycosylation sites and two intramolecular disulfide bonds that are essential for biological functions. Mouse GM-CSF is approximately 54% identical to Mouse GM-CSF. The two proteins show no species cross-reactivity. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF RI+/-/CD116 and the signal transducing common beta chain (CD131) which is also a component of the high affinity receptors for IL-3 and IL5. As an important modulator of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. GM-CSF has been manufactured by recombinant DNA technology and marketed as a protein therapeutic reagent to stimulate the production of white blood cells and thus prevent neutropenia following chemotherapy.

15.6--1000pg/mL

100 uL final volume, dilution factor varies on samples

Mouse ELISA Kits