Mouse IL-21 ELISA Kit

Interleukin-21 (IL-21) is a cytokine encoded by the IL-21 gene in humans. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. The mouse IL-21 is synthesized as a 155 amino acid (aa) precursor protein which consists of a predicted signal sequence and a mature chain. Mature mouse IL-21 shares 59% and 88% aa sequence identity with mature human and rat IL-21, respectively. IL-21 elicits its biological activities by interacting with a heterodimeric receptor complex of the IL-21 specific IL-21R and the I³c (common gamma chain) which is also a subunit of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. In Tfh cells, IL-21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses. It is also required for the migration of dendritic cells to draining lymph nodes. It co-stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation. Dysregulation of the IL-21 signaling has been associated with the development of multiple immunological disorders.

62.5--4000pg/mL

100 uL final volume, dilution factor varies on samples

Mouse ELISA Kits