Mouse VEGF ELISA Kit

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a member of the PDGF family characterized by the presence of eight conserved cysteine residues in a cysteine knot structure and the formation of antiparallel disulfide-linked dimers. Alternatively spliced isoforms of VEGF containing 120, 164, and 188 amino acids (aa) have been found in rats and mice. Mouse VEGF164 shares 97% and 89% aa sequence identity with corresponding regions of rat and human VEGF, respectively. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle, hepatocytes, osteoblasts, neutrophils, macrophages, keratinocytes, brown adipose tissue, CD34+ stem cells, endothelial cells, fibroblasts, and vascular smooth muscle cells. VEGF isoforms are differentially expressed during development and in the adult.

VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (Flt-1) and VEGF R2 (Flk-1/KDR) to activate a series of cellular processes. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells, and modulate blood vessel density and size. In adulthood, VEGF functions primarily in wound healing and the female reproductive cycle.

Sandwich ELISA

100 uL final volume, dilution factor varies on samples

1. Recombinant Mouse VEGF standard: 2 vials

2. One 96-well plate coated with Mouse VEGF Ab

3. Sample diluent buffer: 12 mL - 1

4. Detection antibody: 130 uL, dilution 1:100

5. Streptavidin-HRP: 130 uL, dilution 1:100

6. Antibody diluent buffer: 12 mL x1

7. Streptavidin-HRP diluent buffer: 12 mL x1

8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1

Mouse ELISA Kits