Dengue NS1 Antigen ELISA Kit

This assay employs standard sandwich enzyme-linked immunosorbent assay (ELISA) technology to detect Dengue virus antigen NS1 in the samples. Specific antibody to dengue virus antigen NS1 was precoated in microplate wells. The test samples are incubated in the wells. The Dengue virus antigen NS1 in the sample, if presents, will bind to the immobilized antibody on the wells. Following washing, the anti-NS1 antibody-HRP conjugate is added into the tested wells. After incubation and washing procedures to remove unbound substances, this reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). After terminating the reaction with acidic stop solution, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of NS1 captured in the wells, and to the sample. Colorless wells appear if the dengue virus antigen is negative.

3.5 hrs