Human Calpain-1 Catalytic Subunit ( CAPN1 )

CAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit. CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.

Greater than 90% as determined by SDS-PAGE.

Calpains activity is attributed to two main isoforms: ?-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (?-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sterile Filtered colorless solution.