TransScript® Green miRNA Two-Step qRT-PCR SuperMix

qRT-PCR SuperMix

TransScript® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification.


Highlights
• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.
• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.
• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.
• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).


Applications
Multiple copy and low copy gene detection.


Storage
at -20°C in dark for two years


Shipping
Dry ice (-70 ℃)