Plasmid #4

Plasmid #4 serves as a control; it encodes the CL7 model (CL7M) protein, which directly binds the Im7 protein immobilized on the Im7 Resin. EXPRESSION: Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG: For this plasmid, the target protein serves as the tag and binds directly to Im7. CLEAVAGE SITE(S): A SUMO domain is fused to the C-terminus of the CL7 protein. OTHER TAGS:A Trx tag is upstream of the CL7 protein. EXPIRATION: 6 months from receipt when stored as directed. Plasmid guide with plasmid sequence file is available at Trialtusbioscience.com.