BacMam CT eGFP-CL7-H8

BacMam CT eGFP-CL7-H8 enables target proteins to be labeled with C-terminal eGFP, CL7, and His8 tags. Protease cleavage removes all tags. EXPRESSION:The plasmid is designed for transient expression in mammalian cells or for baculovirus transduction of mammalian cells (BacMam).  Expression is constitutive and driven by the CMV promoter. A Kozak sequence should be added to the 5’ end of the gene insert for efficient translation. AFFINITY TAG:The C-terminal CL7 tag is between the eGFP and His8 tags, and a PreScission protease (PSC) cleavage site is N-terminal to all tags.CLEAVAGE SITE(S):N-terminal PSC P OTHER TAGS: eGFP tag is N-terminal, followed by CL7, then His8. VOLUME: 10 ug at 500 ng/uL EXPIRATION | 6 months from receipt when stored as directed. Plasmid guide is available at TriAltusbioscience.com