FOPflash (Mutant Tcf Binding Sites) (TK-luciferase Reporter) Negative Control

Molecular Biology / MB-Cloning-Plasmids, Vectors

-20°C

Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA.

Transfection grade T cell factor (Tcf) reporter plasmid containing two full and one incomplete copy of the Tcf binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type Tcf binding sites.

Size:
5.5kb

Applications:
Suitable for use in TCF activity assays.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.