Protein Kinase C zeta, Primers, amplimer 680bp (PKCz)

Molecular Biology / MB-Cloning-Primers

-20°C

Contents:
PCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 680bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.

Primer A: Upstream Primer
Primer B: Downstream Primer

Optimal PCR Conditions:
Number of cycles: 35
Denaturation temperature: 94°C for 1 min.
Annealing temperature: 55°C for 1 min.
Extension temperature: 72°C for 1 min.

Storage and Stability:
Store at -20°C in a constant-temperature freezer.