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CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit

CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit

Item no.	ABK-KTB1124-96T
Manufacturer	Abbkine Scientific
Amount	96T
Category	Other Assays & Kits Detection Kits
Type	Assay
Specific against	other
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Alias	6-Phosphofructokinase;PFK; Colorimetric;PFK Assay
Available
Applications Notes	CheKine™ Micro 6-Phosphofructokinase(PFK) Activity Assay Kit provides a simple, convenient, rapid and colorimetric PFK detection method. PFK catalyzed fructosetose 6-phosphate and ATP to generate fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyzed NADH oxidation to NAD+ in turn. The NADH decline rate was measured at 340nm, which reflected the PFK activity
Kit components	•Extraction Buffer • Assay Buffer • Substrate Mix • Enzyme 1 • Enzyme 2
Features & Benefits	• Detect PFK activity in bacterial supernatant of serum (plasma) animal and plant tissues/cells • Detailed sample preparation and results calculation methods are provided
Usage Notes	• Do not mix or substitute reagents or materials from other kit lots or vendors. • It is advisable to measure serum potassium as soon as possible after blood collection. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage Instructions	Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Phosphofructokinase (PFK) is widely found in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructosae 6-phosphate and ATP into fructosae 1, 6 diphosphate and ADP. It is one of the key regulatory enzymes in the glycolysis process. Phosphofructokinase (PFK) activity in bacterial cell supernatant and other samples
Booklet	KTB1124-B.pdf
Tag	PFK
Description	CheKine™ 6-Phosphofructokinase(PFK) Colorimetric Assay Kit provides a simple and easy colorimetric assay for measuring PFK in samples.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96T

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Delivery expected until 10/2/2025

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