CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase（ICDHm）Activity Assay Kit

• Extraction Buffer
• ReagentⅠ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ

• ΔA of Test Tube should be between 0.01-0.3, the sample should be diluted further if ΔA of Test Tube ＞0.3.
• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
• Avoid bubbles while mixing or redissolving components.
• Change pipette tips frequently to avoid cross contamination between components.
• Ensure that all components and equipment are at the proper temperature before starting the experiment.
• All samples and reagents should be on ice to avoid denaturation and deactivation .
• Don't recommend to test too many samples in one time , because enzyme activity is calculated by the variation of the absorbance value per unit time .
• Recommended to calculate the enzyme activity by protein concentration. If you need to calculate by the sample weight , the total enzyme activity is equal to the enzyme activity of the supernatant and pellet which is cytoplasmic extraction.
• Please to extract ICDHm from fresh samples in order to ensure the enzyme activity .
• Please reduce the tissues weight if the OD values is too high . Or reduce the volume of Extraction Buffer if the OD values is too low .

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

KTB1250-B.pdf

CheKine™ Mitochondrial Isocitrate Dehydrogenase（ICDHm) Assay Kit provides a simple, convenient, rapid and colorimetric ICDHm activity detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension) and fungi.