CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit

CheKine™ Micro Acetyl Coenzyme A (Acetyl-CoA) Assay Kit provides a simple, sensitive, rapid colorimetric Acetyl-CoA detection method, which is compatible to various biological samples, especially animals or plant tissues or cell cultures (adherent or suspension). The detection principle is that malate dehydrogenase can catalyze malic acid and NAD to produce oxaloacetic acid and NADH. citrate synthase can catalyze Acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, Acetyl-CoA content is is proportional to the generating rate of NADH . NADH has a special absorption peak at 340 nm, and the calculation of the absorbance value of 340 nm can be obtained the content of Acetyl-CoA.

• simple, sensitive, rapid colorimetric Acetyl-CoA detection method.
• compatible to various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension).
• Provides detailed sample preparation and results calculation methods.

Storage at -20°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.

Acetyl-CoA is widely present in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced during the metabolism of biometrics. In vivo energy substance metabolism is a hinging material. The three major nutrients --glucose, lipid, protein which could be via acetyl coenzyme A to form a common metabolic pathway - tricarboxylic acid circulation and phosphorylation. Through this path, Acetyl-CoA thoroughly could be oxidize carbon dioxide and water, and release energy for ATP synthesis. Further, Acetyl-CoA a is a precursor material while synthetic fatty acid, ketone body , and Cholesterol and its derivatives .

Acetyl-CoA