TAF1L TR-FRET, BioAssay™ Kit (BD1+BD2, TAF1 RNA Polymerase II, TAT Box Binding Protein (TBP)-Associated Factor)

Kits and Assays / Kits-Molecular Biology, BioAssay™

-20°C/-70°C

This kit comes in a convenient format with purified TAF1L (BD1+BD2) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

The TAF1L (BD1+BD2) TR-FRET BioAssay™ Kit is designed to measure the inhibition of TAF1L (BD1+BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, TAF1L (BD1+BD2), substrate, and an inhibitor is incubated for 120 minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Applications:
Suitable for use in screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested.

Kit Components:
White, Nonbinding Corning, low volume, microtiter plate, 1x plate
TAF1L (BD1+BD2), GST-tag, 1x10ug
BET Bromodomain Ligand, 1x50ul
Non-acetylated Ligand 1, 1x15ul
Tb donor, 1x20ul
Dye-labeled acceptor, 1x20ul
3x BRD TR-FRET Assay Buffer 1, 1x4ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.