DNA Isolation Kit, Blood and Serum, BioAssay™

RT

Molecular Biology Grade

The Blood and Serum DNA Isolation Kit is designed for the purification of not only genomic DNA from whole blood or cells, but also genomic DNA fragment (circulating DNA) from serum, plasma, and bio fluid in a spin column format. No phenol-chloroform extraction, no Protease, and no precipitation steps are involved. The sample addition and washing steps can be performed using compatible vacuum manifold, while the lysis of cells and the final elution of the DNA product are performed using a table-top centrifuge.

The blood cells in whole blood or other cultured cells and serum are first lysed in the lysis binding buffer that contains the denaturant guanidine HCl and Triton X100. Ethanol is then added to the samples, which are then added to the filter spin columns. This step facilitates the binding of DNA to the filter matrix. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.

Quality Control:
A representative kit from the same lot is randomly selected for blood genomic DNA isolation from 200ul fresh whole blood. The performance should be as follows:

Yield:
3ug-9ug

Purity:
UV260/280> 1.7

DNA Size:
99% of DNA is > 20kb

Total time of prep less than 25 min

Item
Amount
Storage
1. Lysis Binding Buffer
21ml
RT

2. Wash Buffer 1
26ml
RT

3. Wash Buffer 2
12ml
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4. TE
10ml
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5. spin column set
100 units
RT